In this work, fresh semen from four different races was analyzed, viability, and acrosomal integrity were evaluated. With the objective of assessing the effect of the breed on the quality of freshly preserved dog semen. The races used were Pitbull, Pomerania, Chihuahua, and Creoles. Obtaining as results. The viability of the sperm ranges from very good to regular, with 65% in all three repetitions, in the Pitbull breed with an acrosomal integrity of 75 ± 5% of whole sperm. In the Pomeranian breed, 63.3 ± 12.5% were observed in acrosomal integrity. In the Chihuahua breed, 83.3 ± 5.7% in acrosomal integrity, while in Creole dogs, 71.6 ± was obtained and in acrosomal integrity 71.6 ± 18.9%. In conclusion it can be said that there is no effect of breed on the quality of freshly preserved dog semen.