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Effect of curcuma longa loaded lauric acid lipid vehicle against propionibacterium acnes and its cytotoxic effects on pbmc and hacat

Author: 
Lavanya Balakrishnan, Ganesh Kumar, A., Illanjiam, S. and Karen Kiruba
Subject Area: 
Life Sciences
Abstract: 

Acne vulgaris - a skin disease of the face and chest causing pimples, papules and scarring in adolescent children; has been found to be concomitant with the commensal skin bacteria Propionibacterium acnes. The commonly used drugs for the treatment of acne have many detrimental side-effects. For centuries Curcuma longa and lauric acid have been used in herbal medicines for various ailments including acne. The current study aimed to deliver Curcuma longa through the skin by a lipid vehicle lauric acid to inhibit acne more effectively than Curcuma longa or lauric acid being used as a single drug. Curcuma longa extract was prepared from the dried rhizomes and lauric acid was separated from coconut oil. The lipid vehicle was prepared by Curcuma longa, lauric acid and isopropanol. The Organoleptic properties of the lipid vehicle were studied. The antimicrobial properties of Curcuma longa loaded lauric-acid vehicle, along with the individual extracts of Curcuma longa and lauric acid were studied against Propionibacterium acnes by microdilution broth method. Cell viability assay on Peripheral Blood Mononuclear Cells by Dye Exclusion Method and the Cytotoxicity on HaCaT cell line by MTT assay were studied on the Curcuma longa loaded lauric-acid vehicle, as well as with the individual extracts of Curcuma longa and lauric acid.It was found in the study that the combination of Curcuma longa and lauric acid in a lipid form proved to be more beneficial than the individual extracts.

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