The present study was to carried out plant regeneration of Arachis hypogaea from in mature leaf explant was cultured on MS medium containing various concentration of IAA (2.5mg/l) and BAP (1mg/l) was maximum percentage of callus induction from shoot explants. KIN (2.5mg/l) and IAA (1mg/l) was maximum shoot regeneration of callus. BAP+ KIN (2.5mg /l) in combined effect of 1AA (1mg/l) results showed maximum yield of callus and shoot induction. IBA (3.0 mg/l) and KIN (0.5mg/l) was produced amount of maximum number of root induction. The present study to find out to the various concentrations of hormones was vital role of callus induction, shoot regeneration and root induction.