Effect Of Omega-3 Fatty Acid On Lead Induced General And Behavioral Profile In Male Wistar Rats: An Experimental Study

Aims and objective: The present study was aimed to see the effects of omega-3 fatty acid on lead induced general and behavioral profile in wistar rats. Materials and Methods: 32 Male wistar Rats (weight 180±20 g) were acclimatized for 1 week prior to experimental use and after that divided into four groups of 8 rats each. Group 1 served as control, treated with distilled water, p.o., daily for 90 days, Group 2 treated with lead acetate (3mg/kg body weight, p.o., daily for 90 days), Group 3 treated with omega-3 fatty acid (300mg/kg body weight, p.o., daily for 90 days) along with lead acetate (3mg/kg body weight, p.o., daily for 90 days). However, Group 4 treated with Vitamin E (100mg/ kg body weight, p.o., daily for 90 days) as standard drug along with lead acetate (3mg/kg body weight, p.o., daily for 90 days) . A set of five rats randomly selected from each treatment group was observed general and behavioral studies 24 h after the last dose of treatment. A base line body weight, food, water and lead intake was recorded after that general and behavioral studies were performed Statistical analysis: The data was expressed as the mean±S.E. and was analyzed by one way analysis of variance (ANOVA), involving Newman-Keuls test for post-hoc comparisons. The level of significance was accepted at p<0.05. Results: The results of the effect of administration of omega-3 fatty acid on lead induced general and behavioral profile in rats were seen. The body weight was significantly reduced in Pb-exposed group as compared to the other groups. In the experimental period, food intake, water intake and lead intake was significantly decreased in the Pb-exposed group as compared to the control groups and we observed that general and behavioral profile were not significant among the groups. The results obtained were compared with vitamin-E (100mg/kgb.wt.), the standard drug as antioxidant. Conclusion: Our data suggested that omega-3 fatty acid significantly decreased the adverse effects of lead exposure on male wistar rats.