Effect of urine samples of experimental rats on the growth of calcium oxalate crystals in-vitro

Urinary calculi formation is a very complex phenomenon taking place in a step-wise manner leading to urinary stone disease. Formation of urinary crystals is an essential step in stone disease. Common known stone components are calcium oxalate, calcium phosphate and uric acid. In- vitro studies was done by growing crystals in silica gel medium. Urine samples of the non-diabetic non-calculogenic, diabetic non-calculogenic, non-diabetic sodium oxalate induced calculogenic, non-diabetic ethylene glycol induced calculogenic, diabetic sodium oxalate induced calculogenic and diabetic ethylene glycol induced calculogenic were collected and added in requisite amount on the top of the gel. Length of the crystal column and size of the crystals were noted on days 1, 7, 14, 21 and 30 respectively. Comparative study of the mean length of crystal column in- vitro showed the maximum crystal growth in non-diabetic ethylene glycol induced calculogenic rat urine added set. Comparative study of mean length of crystal column between the diabetic non-calculogenic and diabetic calculogenic showed maximum crystal growth in the diabetic calculogenic rat urine added set. Analysis of Variance showed statistically significant difference in mean values among the groups and the size of the calcium oxalate crystal in- vitro showed significant difference in mean (p=0.001). Highest mean of 398.75+1.25µ was seen in the diabetic sodium oxalate induced calculogenic rats. Duncan’s Multiple Range Test showed maximum crystal size in the diabetic sodium oxalate induced calculogenic rats compared to other groups. In- vitro growth of calcium oxalate crystals was maximum in the diabetic urine added set. This indicates the tendency of diabetes along with calculogenesis for promoting maximum crystal growth.