Major enzyme activities of vermicompost of papermill sludge by earthworm species Eudrilus eugeniae and its simple compost were assessed across different periods in relation to their respective initiative substrates. In this experiment, the vermibed was prepared from different raw material viz., Mixed Liquor Suspended Solids (MLSS) and Leaf Litter (LL) were mixed with Cow Dung (CD) mixed with different ratios. All vermibeds expressed a significant increase in enzyme activities. Overall, the maximum value of enzyme activities was received in CD + MLSS + LL in 1:1:2 ratios. Further, these differences in overall enzyme activity agree with the variation found in extracellular enzyme activity suggesting certain dependence on substrate availability.