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Estimation of genetic diversity in lentil (lens culinaris) using protein profiling and rapd

Author: 
Sheikh, G.N., Showkat Ahmad and Rajdeep Kudesia
Subject Area: 
Life Sciences
Abstract: 

Electrophoretic SDS-PAGE and RAPD analysis were performed to established genetic diversity for five accessions (IPL-406, IPL-81,DPL-62, DPL-15, & Sehore-74-3) and to elucidate their genetic relationships. The resulted protein banding pattern showed high level of polymorphism( i.e., 95.6% and could be used to discriminate completely among the five accessions under study. However, the protein profile could be considered as general biochemical finger print of the lentil.During present study the polymorphism was high at protein level. One decamer primer(OPA-17) having sequence CACCGCTTGT was used to estimate genetic diversity in Lens culinaris accessions.Atotal of 43 bands were scored , 39 bands showed polymorphism( 90.6%) and the rest 4 bads showed 9.30% monomorphism during RAPD analysis. Number of bands produced in each accession varied during RAPD and SDS-PAGE analysis .Similarity index reveals maximum similarity between accessions IPL-406(L-1) and DPL-15(L-4) , DPL-15(L-4) and Sehore-74-3(L-5) i.e., 35.71% and 35.29% respectively while distantly related accessions were IPL-81(L-2) and DPL-62(L-3) with similarity index 23.8% when RAPD profile was observed but when protein profile of five accessions of lentil was observed, show highest similarity index value between accessions IPL-406(L-1) and Sehore-74-3(L-5) i.e., 36.6% while distantly related accessions show lowest similarity index were IPL-406(L-1) and DPL-15(L-4) i.e., 15%. Two dendrograms constructed based on Jaccard’s method and UPGMA using oth SDS-PAGE and RAPD profiles. The resulting dendrogram for protein profile revealed two main seperate genetic clusters ; the first comprises the accession IPL-81(L-2), The first cluster was further subdivided into IPL-406 and Sehore-74-3 while second cluster comprises accessions DPL-62 and DPL-15.The dendrogram constructed for RAPD profile revealed main separate genetic clusters ; the first cluster comprises the accession DPL-62, the first cluster was further subdivided IPL-406 and DPL-15 while as second cluster comprises accession IPL-81 and Sehore-74-3.

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