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Evaluation of in angio-suppressive activity of the biophytum sensitivum (bark) extracts by chorioallantoic membrane (cam) assay

Author: 
Manisha Mhaske and Ghanshyam Gonjari
Subject Area: 
Life Sciences
Abstract: 

Angiogenesis is the formation of new blood vessels from pre-existing vessels, capillaries and post-capillary venules. It plays an important role in many normal physiological processes such as normal tissue growth, embryonic development, wound healing and menstruation. Many natural inhibitors block the process of angiogenesis which may leads to inhibition of tumor growth. The chick chorioallantoic membrane (CAM) assay is well vascularized in vivo model used to study angiogenesis, anti-angiogenesis and teratogenic effect of individual compounds and complex plant extracts. In the present investigation angio-suppressive activity of B. sensitivum (bark) in acetone extract is evaluated by chick chorioallantoic membrane (CAM) assay. The fertilized eggs of Gallug gallus (murghi) was incubated at 380C with relative humidity. The embryos of 48, 72 and 96hrs CAM were exposed to 1mg/ml acetone extract of B. sensitivum(bark) and were further incubated upto 144hrs and CAM was studied. The present investigations showed angio-suppressive effect of B.sensitivum (bark) on the number and area of tertiary vitelline veins (TVV).

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