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Evaluation of antibacterial efficacy of ozonated olive oil and cold pressed neem oil against enterococcus faecalis: an agar well diffusion study

Author: 
Dr. M.V. Pratyusha, Dr. Jayalakshmi, K.B., Dr. Prasannalatha Nadig, Dr. Sujatha, I., Dr. Arul Selvan K and Dr. Zaharunnissa
Subject Area: 
Health Sciences
Abstract: 

Background: Successful endodontic treatment depends on effective disinfection and complete sealing of root canal. Various medicaments are advised for disinfecting root canal, such as herbal and non-herbal medicaments. Aim: This in vitro study was done to evaluate the antibacterial effect of ozonated olive oil and cold pressed neem oil against Enterococcus faecalis (E. faecalis) (ATCC 29212). Materials and Method: Agar well diffusion method was used to evaluate the antibacterial action of Group 1: Ozonated olive oil, Group 2: cold pressed neem oil, Group 3: 2% Chlorhexidine, Group 4: Calcium hydroxide and Group 5: Normal saline. The petri plates were incubated aerobically at 37o for 48 hours. The inhibition zones against E. faecalis were recorded. The data was statistically analyzed using one way analysis of variance (ANOVA) and Post Hoc test (p< 0.05). Results: Ozonated olive oil showed the maximum mean value for the zone of inhibition after 48 hrs followed by 2% CHX and then cold pressed neem oil. Least mean value for the zone of inhibition was obtained for calcium hydroxide. There was a statistically significant difference between the groups. (p<0.001) Conclusion: Ozonatedolive oil was most effective against E.faecalis when compared to other experimental groups and can be used as an alternative intracanal medicament.

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