Pollution of ground and surface waters by nitrates is a widespread and serious global problem. The objective of this study was to evaluate of nitrate and nitrite in drinking water of some rural areas of Shahrekord, Iran. We collected 490 drinking water samples from49 villages in the rural areas of Shahrekord, Iran and analyzed them for NO3-N and NO2-N. Nitrate and nitrite ions were analyzed by Spectrophotometer 2100 UV-Vis, at 220 and543nm. The highest (29.94±4.2 4mg/L) and lowest (9.82±2.6 mg/L) concentrations of nitrate in duration 5years were belonged to Sartashnez and Qaraqush, respectively. The highest (0.069±0.004mg/L) and lowest (0.005±0.001mg/L) concentrations of nitrite in duration 5years were belonged to Shurab-eShagir and Salm, respectively. The results obtained for the nitrate and nitrite indicated that the 49 areas are below the limits allowed by Iran and WHO Standards.