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Evaluation of uh400 inducer for adapted and inducing haploid ability in vietnamese condition

Author:

Vu Van Liet, Nguyen Viet Long, Nguyen Van Ha, Pham Quang Tuan, Hoang Thi Thuy, Vu Thi Bich Hanh, Tran Thi Thanh Ha, Nguyen Thi Nguyet Anh and Le Thi Kim Hue

Subject Area:

Life Sciences

Abstract:

Adapted evaluation of the UH400 line in the Northern of Vietnam with ten seasons from 2012 to 2015, it could be maintained and multiplication in spring and Autumn-winter season and sowing time for most suitable from early to middle of February annual. UH400 line can inducing the inbred lines with ratio from 4 to 10 percentage depends on the genotype, seeds of haploid type identified by the morphological marker R1-nj, this was confirm by molecular marker SSR. Doubling haploid lines, which were induced by colchicine with concentration was 0.06%, were obtained about 50 percentages of the survived DH lines. These DH haploid lines were evaluated on the field trial together inbred lines in Autumn-winter season in 2015. They showed appropriated growth and development in Northern of Vietnam and agronomical characteristics were indifferent compared within bred lines, they have yield vary from 14.21 to 44.12 quintal/ha. These DHs could be used for hybrid maize breeding programs in Vietnam.

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