Evaluation of valli panchmoola through hydro suspension method – new technology

Background: Suspension microscopy is a scientific way to observe the suspended matter in three parts. Concept arises from fresh Hima preparation. Normally any liquid preparation forms many layers after keeping sometime, with different qualities. Each and every layer consist different physical form, chemical form, organoleptic form and also at the micro level. Hydro suspension preparation forms different layers. If improper mixture is taken, Scientifically there may be a loss of active constituents in lower and upper layered mass i.e. tannins, oils, fibers, etc. Objectives: Scientifically, the light weighted dusting particles occupy a top most upper layer, the heavy particles settled down at the bottom layer. The consumer consumes only the middle portion. There may be a loss of many cellular constituents. To overcome from this, the problem creates new concept of Hydro suspension microscopy to know the constituents which are present in the different layers. Method: 5 g of both the compositions were taken individually and soak in 50 ml water in a measuring cylinder for 24 hrs in undisturbed condition. The next day, with the help of dropper, the microscopy of three different layers i.e. upper, middle and lower layers from both the compositions were done. Result: Rhomboidal, Cluster and Rosette type of crystals, cork in surface view, etc. in upper layer. Middle layer shows annular vessel, parenchyma cell, etc. and lower layer shows collenchyma cells, simple fibre, etc. Conclusion: Most of the constituents are settled down and very less portion of the drug is present at the middle and upper portion. In the microscopic study, the quantity of brown content, fibres and starch grain was high at the lower portion. The crystals and vessels were present at the upper and middle portion.