The extraction and characterization of crude oil of Moringa oleifera oil found in India, Tamil Nadu has been carried out. Normal hexane was used as solvent for the extraction process. The oil produced was refined through transesterification process using methoxide and magnetic stirrer local. The characterization analysis revealed that tested parameters, which include specific gravity, refractive index, viscosity, density, acid value, saponification value and iodine value for both crude and refined Moringa oil produced, were within the ASTM standard specifications. The oil is of good quality and could be recommended suitable for industrial usage.