Genetic variation among the scabies-infested sweet potato cultivars

Sweet potato tuber posses various nutritious content makes them fit for food source, whether consumed as the whole, or their derivatives. These plants are susceptible to diseases including scabies. Current study was aimed to show polimorphism patterns of 10 different scabies-infested sweet potatto cultivars using 10 RAPD-PCR primers. For this purpose, 10healthy individual sweet potato plants were initiallyinfested with the Sphaceloma batatas spores then isolated for their DNAs using a modified CTAB.Current data showed the DNAs ranged from 61.5-1011 ng/ml, but contaminated witheither protein or polyphenol. Six primersresulted 100% polymorphism on the 10 cultivars with different total numbers of loci. The highest polymorphismsappeared on OPA-11 which showed 12 loci with 35 bands, while the highest number of bands were 48 bands was produced by OPA-3 with 8 loci. Other primers, however, showed 1-3 loci monomorphic. The cultivars might be grouped into four major groups and two sub groups within group I and III. The Borobudur and Sari cultivars were closely related with genetic distanceof 0.181.