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Green synthesis of silver Nano particles using leaf extract of Coccinia indica and its activity against multiple drug resistant (MDR) strains of bacteria

Author: 
Mili Thakkar, Tumane, P. M., Bhandari, P. R. and Durgesh D. Wasnik
Subject Area: 
Life Sciences
Abstract: 

This study evaluate antibacterial property of green synthesized silver nanoparticles Coccinia indica extract as reducing agent against multiple drug resistant bacteria (MDR) such as E. coli, S. aureus and Pseudomonas aeruginosa. Silver nanoparticles were synthesized through Coccinia indica extract and characterized using UV-VIS spectroscopy, SEM, TEM techniques. The antibacterial activity assays were done against all bacterial pathogens by well diffusion method. The diameter of silver nanoparticles was predominantly found within the range 100-300 nm. The absorption peak at 420 nm broadens with increase in time indicating polydispersity nature of the nanoparticles. The present study showed that P. aeruginosa and E.coli was found to be resistant against Ampicillin, Cephotaxime, Ceftazidime, Clotrimazole and Gentamycin. S. aureus was resistant toward Methicillin, Vancomycin, Imipenem and Tobramycin. In this study, the Green SNPs showed excellent zone of inhibition against Pseudomonas aeruginosa (22 mm) whereas least zone of inhibition was found against S. aureus (8 mm). It has been found that nanoparticles treated with herbal solutions at different concentrations resulted into inhibition of S. aureus at 12.5 µg/disc and slightly increased concentration of SNPs i.e. 1.56 µg/ml is the minimum concentration found inhibitory to the growth of MDR strains of Pseudomonas whereas SNPs didn’t show any zone of inhibition against E. coli. The present findings suggest that green synthesis of nanoparticles are simple, quite effective method that can be used as an alternative antibacterial agent against diseases caused by multiple drug resistant pathogens.

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