Identification of anti MRSA (Methicillin resistant Staphylococcus aureus) compounds from Ethanolic leaf extracts of Aegle marmelos by GC - MS

Due to increase in occurrence of antibiotic resistant bacterial pathogens, there is a pressing need to develop effective alternate antibacterial compounds. Medicinal plants are the rich source of secondary metabolites that serve as potential antibacterial agents. The aim of the present study is to fractionate screen the crude ethanolic leaf extract of Aegle marmelos screen for the antibacterial activity against Methicillin resistant Staphylococcus aureus (MRSA) and identify the compounds present in the active fraction. Column chromatography of the crude ethanolic extract yielded a total of 10 fractions. One candidate fraction showed highest antibacterial activity. Gas chromatography mass spectral studies identified the presence of seven phytochemical compounds in the active fraction. Among them, the three leading compounds are alphamonostearin followed by stigmast–5–en–3–ol and pyrrolidino piperazine 3, 6 dione.