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Identification of hindiii polymorphisms in the two intron of ghr gene in native Iraqi chicken

Author: 
Muhannad M. AL-Arekabi, Eman H.AL-Anbari, Iman H. AL-Dulaimi, Hameed A. AL- Ziaidi and Yousif O. AL-Dreesaoy
Subject Area: 
Life Sciences
Abstract: 

The gene of the growth hormone receptor has played a great role in the chicken growing and reproduction. Polymorphisms for this gene were several variations in the animal genome, could be used as genetic marker for growth selection and economic traits. The purpose of this study was to identify polymorphisms in Growth hormone receptor in Iraqi local chicken .we collected 95 blood samples form Iraqi Native chicken (47 samples from the Black feather native fowls and 48 samples from the Nicked neck native fowls) to inspect growth hormone receptor gene polymorphism using PCR-RFLP technique. As stated to the data, HindIII enzyme produced 2 final alleles R and G. the R allele was placed in 290 and 428 bp while G allele placed in 170, 258 and 290 bp. Also, RR genotype stracture was in 290 and 428 bp while GG placed in 170, 258 and 290 bp, respectively. Besides, in this research no heterozygote identified. As it was seated in the data, there was significant difference (P>0.01) among polymorphism in intron 2 gene in growth hormone receptor. The consequences of current study showed that the intron 2of GHR is polymorphic in Iraq native fowls and could be exploited as a genetic marker for selection programs of growth-related traits.

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