Lactic acid bacteria play an important role in a large number of various traditional dairy products. This study investigates isolation and identification of some lactic acid bacteria from Domiate cheese. Six isolates were selected and identified; Domiate cheese isolates had an inhibition and bactericidal effects on the growth of some pathogenic microorganisms as Staphylococcus aureus; E.coli ATCC 25922 and Bacillus subtilis NCIB3610. The isolates had antibacterial effect in deferent degree. Lactobacillus acidophilus CH5 isolate was the best effect on the three indictor strains.