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Immobilization of phaseolus vulgaris amylase onto chemically charged woven bombyx mori silk fabric

Author: 
Kirti Rani and Swapnil Saxsena
Subject Area: 
Life Sciences
Abstract: 

Amylases are starch degrading enzymes. They are widely distributed in microbial, plant and animal. They degrade starch into free glucose and limit dextrins. Hence, amylases have couple of applications in food & pharmaceutical industries too. Amylase extracted form Phaseolus vulgaris was immobilized on a Bombyx mori silk fabric activated by chlorination and diazotization. The 82 % of immobilization was done onto chemically charged Bombyx mori silk fabric. The optimum temperature, pH, time of incubation, substrate concentration and effect of calcium chloride concentration were studied. Thermal stability of enzyme was improved after immobilization up to 60oC which was 50oC for free enzyme. As well as immobilized enzyme was stable at least for 4 -5 months when stored at 1 M KCL solution at 4º C.

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