An experiment was conducted at Botany Section, College of Agriculture, Nagpur (MS) India during year 2014-2015 to study the influence of putrescine and IBA (50, 75,100,125, 150 ppm each) on chemical, biochemical, yield and yield contributing parameters of soybean. Experiment was conducted in Randomized Block Design. Considering the treatments under study two foliar sprays of 100 ppm putrescine and 100 ppm IBA at two stages ie., before flowering and 10 days after flowering were found to be most effective in improving nitrogen, phosphorus, potassium, chlorophyll content in leaves and protein and oil content in seeds. Yield contributing parameters viz., number of pods plant-1, weight of 100 seeds were also significantly enhanced. Seed yield ha-1 was also significantly increased by 100 ppm putrescine (25.89%) and 100 ppm IBA (20.34%) over control.