Influence of temperature on production of polygalacturonase, polymethylgalacturonase and cellulase enzymes by alternaria solani in vitro

An attempt was made to find out the effect of different temperatures ranging from 150 C to 450 C on production of polygalacturonase (PG), polymethylgalacturonase (PMG) and cellulase (Cx) enzymes by Alternaria Solani in vitro. The production of polygalacturonase (PG), polymethylgalacturonase (PMG) and cellulase (Cx) enzymes has been recorded between wide range of temperature, i.e. between 150 C to 350 C. At 150 C temperature, the production of these three cell wall degrading enzymes were found to be very low to which increases gradually with the increase in the temperature upto 280 C. The 280 C was found to be the best and favourable temperature for maximum production of these enzymes. Above 350 C, further higher temperature, i.e. 400 C and 450 C have been found to detrimental for the production of all the three enzymes as no trace of polygalacturonase (PG), polymethylgalacturonase (PMG) and cellulase (Cx) enzymes has been detected in culture filtrates.