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Intra-species protein variation studies on dolichos lablab linn

Author: 
Sachin Palekar, Behnaz Patel, Vinaya Rane, Digvijaysinh Chauhan, Ajeet Patil and Shreya Patwardhan
Subject Area: 
Life Sciences
Abstract: 

Legumes are a source of wholesome protein, alimentary fiber, and bioactive substances displaying antioxidant activity together with anti-inflammatory and antineoplastic properties. Cereals and Pulses form an integral part of the human diet.Eight accessions of Dolichos lablab Linn. were procured from Pulses and Castor Research station, Navsari. Total proteins were extracted in chilled 100 mM Tris-HCl buffer (pH 8) containing 1% SDS and 0.01% - mercaptoethanol. Albumins and Globulins were extracted based on differential solubility in water and dilute salt solution respectively. The genotype of an organism expresses itself through the phenotype - through proteins. So, protein profiling of the plant is essential to reveal the role of proteins. Electrophoresis serves as an imperative tool for studying the protein profiling of the plants. Therefore, Extracted samples were subjected to electrophoretic separation by SDS-PAGE. Proteins were stained using Coomassie blue and silver staining technique. The binary data was subjected to Statistical analysis using MVSP software. The genetic similarity matrix was calculated using Jaccard’s co efficient and the dendrogram was based on Jaccard’s distance matrix is obtained using UPGMA. Thus three different Phylogeny trees for 8 accessions of D. lablab L. were made based on protein profile of Total Proteins, Albumins, and Globulins. Present work provides the protein variation and phylogenetic relationships between eight accessions of Dolichos lablab L.

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