Using the random amplified polymorphic DNA (RAPD) method low molecular diversity was detected among D. tritici-repentis isolates recovered from wheat leaves collected in different locations in Georgia. The most of isolates were of highly similar. Similarity index varied between 0.333 -0.944. Maximum index was 0.944 for isolates A1a and A1b, low index (0.167-0.444) was seen for 23 pairs. High degree of genetic similarity was found among isolates obtained from Dusheti region and among Sachkhere isolates. The dendrogram of PARD data contains two groups. The grouping by DNA polymorphism showed no association with the geographic origin of the isolates.