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Laboratory evaluation of the sd bioline- dengue duo rapid immunodiagnostic test kit for detection of ns-1 antigen and igm antibody during dengue outbreak in Delhi

Author: 
Kumar S Abhishek and Anita Chakravarti
Subject Area: 
Health Sciences
Abstract: 

Background: Dengue a mosquito borne viral fever is caused by Dengue virus of family Flaviviridae. It has a wide spectrum of sign and symptoms ranging from acute self-limiting infection to fatal complications in few cases like Dengue Hemorrhagic Fever (DHF), Dengue Shock Syndrome (DSS). Several Rapid immuno-chromatographic and ELISA based diagnostic kits are available for laboratory diagnosis in clinically suspected cases. Due to threat of developing fatal complications, rapid diagnosis becomes important in order to prevent such complications. Objective: Evaluation of Rapid immuno-chromatographic test kits taking ELISA (NS-1 Ag and IgM Antibody) as reference. Materials and Method: Cross-sectional study. ELISA and SD BIOLINE- Dengue Duo Rapid immunodiagnostic test kit as per manufacturer’s instruction. Result: Sensitivity and Specificity for NS1 antigen 89.6% and 98% and for IgM antibodies 94.7% and 100% respectively were observed using SD BIOLINE- Dengue Duo Rapid immunodiagnostic test kits taking NS-1 antigen and IgM anti-dengue antibody ELISA as reference. Conclusion: During an outbreak where rapid diagnosis is needed, rapid diagnostic kits are good alternative to ELISA based tests.

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