The present study was carried out to establish the properties of leaf extract of Vitex negundo was tested for larvicidal activity against early 4th instar larvae of Culex quinquefasciatus mosquito using standard WHO technique. The mortality rate was observed at 5 to 200 ppm, after 12, 24, 36 and 48 hrs of the treatment. And no mortality rate was observed in control. The methanolic extract of V. negundo was found effective and 100% result was observed in this treatment. This plant extracts are easy to prepare, inexpensive, and safe for mosquito control which might be used directly as larvicidal agents in small volume aquatic habitats or breeding sites of around human dwellings.