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Method devlopment and validation of gallic acid and ellagic acid in argwadharistam

Author:

Paramita Das, Dr. T. Panneerselvam, Dr. Padmavathi. P. Prabhu and Jithendar Reddy

Subject Area:

Health Sciences

Abstract:

A novel RP-HPLC method has been developed for the estimation of Gallic acid and Ellagic acid in Argwadharistam, which is popular herbal formulation. It is an ayurvedic formulation made out by Terminalia Chebula along with other ingredients. Studies have shown that it has used in all types of dermatitis. The use of gradient elution of Acetonitrile & buffer solution enabled the efficient separation of Gallic acid and ellagic acid in 4.8 min and 8.46 min (Rt), five calibration standards in range of 2.0-10µg/ml for Gallic acid and 10-50µg/ml for Ellagic acid at 254nm quantification was effected at 254nm. Validation was performed according to ICH guidelines. Proposed method showed good linear correlation coefficient (r2=0.999). The LOD and LOQ of Gallic acid and ellagic acid were 0.8µg/ml & 2.5µg/ml and 1.5µg/ml & 5µg/ml respectively. The recoveries are between 98 and 102%. The developed and validated method was simple and accurate can be used for the estimation of Gallic acid and ellagic acid in herbal formulation.

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