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Micronuclei induction in different tissues of Euphlyctis cyanophlyctis (common skittering frog) exposed to Sublethal concentration of mercuric chloride

Author: 
Kour Preetpal, Tripathi, N. K. and Poonam
Subject Area: 
Life Sciences
Abstract: 

In the present study acute toxic effect of mercuric chloride was carried out in three different tissues (RBCs, Intestine and kidney) of skittering frog (Euphlyctis cyanophlyctis). The study was conducted using the micronucleus test. The frogs were treated with four different sublethal concentrations of mercuric chloride (1.5µg/kg, 3.0µg/kg, 6.0µg/kg, 12.0µg/kg) after calculating the LC50 value which was found to be 18µg/kg. Each dose was injected intraperitoneally once in the treatment period for 24, 48, 72 and 96hrs to study its potential toxic effect. It was observed that this heavy metal treatment induced a significant increase in frequency of micronuclei at different concentrations in frog for 24, 48, 72 and 96hrs when compared with the control. The results lead us to the conclusion that there is a dose and time dependent induction of micronuclei frequencies in different somatic tissues of the frog due to genotoxic and cytotoxic properties of this heavy metal.

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