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Modified verhoeff's technique for staining of elastic fibers

Author: 
Mr. Ziyad Mudasir and Mr. Ahmed Ibn Edriss, M.
Subject Area: 
Health Sciences
Abstract: 

Introduction: Reliable identification of elastic fibers structures in light microscopy can be altered by several diseases which cause changes in elastic tissues, such diseases are ageing and atrophy caused by arteriosclerotic changes. Numerous empirical methods for elastic fibers displaying were evolved and modified along based on chemical structure of the elastic tissues components A number of techniques can be applied for elastic fibers demonstration these techniques can stain the fibers intensely but sometimes being unrelated. Study design: This is an experimental descriptive study conducted in White Nile State (Sudan), at University of El-Imam El-Mahdi (Kosti) from 27th April 2017 to 14th June 2017. Methodology: Twenty arterial biopsies were taken from a rabbit after anesthesia. All biopsies were 1 × 1× 0.3 cm in dimension. After the specimens were collected, all of them were immediately fixed in a wide suitable container by 10% neutral buffered formalin ten times the size of specimen for 48 h. After fixation of specimens, the cut- up was done, specimens were put in cassettes then bearded the unique cases number. The specimen then passed into a tissue processing machine (Leica, 2000) for further treatment in Table 1. After tissue processing was completed the blocks containing the specimen was thereafter subjected to routine section cutting. 20 blocks were prepared. The blocks were cooled to solidify to turn out their moulds and were then cut by rotary microtome (Diapath Galileo, fully automatic microtome Galileo, 2012). 10 sections of 5 μm thickness were sectioned from each block. 100 tissue section were stained by original Verhoeff's Van Geison method as control and the remaining 100 tissue sections were stained by modified Verhoeff's method. Results: Elastic fibers were stained brown in a yellow background by modified Verhoeff's method. Conclusion: Modified Verhoeff's method is easy to perform in a few steps (no need for removing of iodine pigment). The differentiation step can be carried out even by less experienced person and give good results.

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