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Molecular Characterization Of Sugarcane Genotypes For Their Salinity And Susceptibility Using Trap Markers

Author: 
Forough Jomhe Farsangi, Avinash S. Thorat and Rachayya M. Devarumath
Subject Area: 
Physical Sciences and Engineering
Abstract: 

Sugarcane is a cash crop in India and salinity affects its productivity and crop growth. Identification of molecular markers linked to salinity tolerance traits has provided plant breeders a new tool for selecting cultivars with improved salt-tolerance and hence, molecular characterization of eighteen sugarcane genotypes of salinity tolerant and susceptible was carried out using thirty combinations of 5 TRAP markers related salinity tolerant ESTs and 3 arbitrary primer combinations. Out of 124 alleles, 81 (65%) were found polymorphic and The PIC values were ranged from 0.10 to 0.41 with an average of 0.30 furthermore Rp value varied from 0.33 to 4.33 with an average of 1.48. Also, the range of polymorphism was found about 25% to 100%. The genetic similarity coefficients ranged from 0.53-0.91 with an average of 0.72 which revealed the existence of limited genetic variation among 18 sugarcane genotypes. Abbreviations: ESTs: Expressed sequence tag MA: Monomorphic Alleles P: Percentage Polymorphism PA: Polymorphic Alleles PCR: Polymerase chain reaction PIC: Polymorphic Information Content PIC: Polymorphism Information Content RAPD: Random Amplified Polymorphic DNA Rp: resolving power TA: Total Alleles TM: Temperatures of primers TRAP: Tartrate-resistant acid phosphatase

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