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Molecular phylogenetics of global and indian mosquito species in terms of bioinformatics and biostatistics

Author: 
Divya Damodaran, Dr. Sudarsanam, D., Maria Infanshia Sajini, Vasantha Suppriya, Joel Singh, D. and Siddhardha Solosan
Subject Area: 
Life Sciences
Abstract: 

Recent studies on DNA-based approaches show a promising trend in the rapid description of biodiversity. Traditional morphology-based assessments are timeconsuming and require specialists whose numbers are insufficient and dwindling. A DNA-based method called DNA barcoding has been proposed as a rapid means of cataloguing species. Hebert et al., (2003) specifically suggest the employment of DNA sequences as taxon ‘barcode’s and propose that the mitochondrial gene cytochrome oxidase I (COI) serves as the core of a global bio identification system for animals. The unique contribution of DNA barcoding to mosquito taxonomy and systematic is a compressed timeline for the exploration and analysis of biodiversity. According to Knowlton and Weigt (1998) among the mitochondrial genes, cytochrome oxidase subunit 1 (COI) is to be the most conserved gene in the amino acid sequences and hence has distinct advantage for taxonomic studies. The morphological identification keys used currently for identification of mosquitoes are mainly related to imaginal and fourth instars only. Hence, difficult to identify other stages of development collected in the field, if not reared in the laboratory.

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