Mosquitoes play a vital role in the spread of vector borne diseases and their management has gained great importance. Plant extracts have been studied for their mosquitocidal activity against various vectors. The present study was found on the larvicidal and pupicidal activity of Lantana camara (L) essential oil and azadiractin loaded novel nanoemulsion formulation for the control of vector mosquitoes. Nanoemulsion formulations of double emulsion type- W/O/W. Lantana camara (L) essential oil and azadiractin loaded nanoemulsion formulation. Five different concentrations (5, 10, 15, 20,25ppm) were prepared and the insects (Culex quinquefaciatus and Aedes aegypti) were treated with it. The mortality percentage and lethal dose (LC50) were calculated. The study clearly reduced the repellent activity of the oil emulsion (Lantana camara (L)) and azadiractin loaded nanoemulsion formulation had potent biocidal activity on vector mosquitoes.