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network pattern changes of platelet rich fibrin in different age groups and gender: a cell block cytology study

Author: 
Dr. Triveni Kale, Dr. Varsha Ranmare, Dr. Govind Bhartiya, Dr. Mitali Thamke and Dr. Rutuja Sankhe
Subject Area: 
Health Sciences
Abstract: 

Background: Regeneration of the destroyed periodontal tissues is the ultimate goal of periodontal therapy. The factors that affect fibrin formation and structure may be: Genetic factors, acquired factors and other parameters including the age and gender of the patient. The patterns and arrangement of fibrin networks within the PRF clot, their capacity to entrap platelets and wbcs and the impact of age and gender of the patient on fibrin network patterns and arrangement have not been highlighted till date. Aims: The study is to evaluate the variations in the fibrin network patterns of the PRF clot, isolated from individuals of different age groups and gender. Materials and method: Ninety patients were divided in three age groups with equal gender distribution. PRF was prepared from blood samples of all patients and were subjected to cell block cytology method of histological analysis and slides will be prepared to histologically assess the age and gender related changes in (i) Fibrin network patterns in terms of density and (ii) Entrapment of platelets and white blood cells (wbcs) within fibrin meshwork. Result: In males and younger individuals more dense pattern seen compared to females and old age. Furthermore, variation in a number of platelets and wbcs entrapped within fibrin network in relation to age was noticed. Conclusion: So the age and gender can be one of the important factor on quality of PRF in terms of fibrin network pattern and hence, platelet and WBC’s entrapment within these fibrin network.

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