The present investigations were undertaken on laboratory studies of Trichogramma chilonis (Ishii) during the year 2013-2014 in the Bio-control laboratory, Department of Agricultural Entomology, College of Agriculture, Dapoli (Maharashtra).The results of effect of different hosts on parasitisation potential of T. chilonis revealed that the per cent parasitisation and per cent adult emergence of T. chilonis was found maximum on S. litura eggs followed by C. cephalonica and H. armigera eggs.