Pathogenic action of cx, pg and pmg enzymes of fusaium udum and fusarium oxysporum f.sp.ciceri

Wilt disease affects the pulse crops at greater extent mainly due to fusarium species. Fusarium species releases pectolytic and cellulolytic enzymes responsible for disintegration of middle lamella and matrix of cell wall of particular host crop and infect them. Present study mainly use to calculate the role of cellulase(Cx) ,polygalactouronase (PG) and pectin methyl galacturonase(PMG) in fusarium for causing wilt disease. Result shows that with increasing incubation time, the activity of all three enzymes have been enhanced. Polygalacturonase shows highest activity i.e. 24.1 in case of fusarium oxysporum f.sp.ciceri and 38.7 in case of fusarium udum at 90 minutes time interval. While cellulase shows lowest activity 4.8 in case of fusarium oxysporum f.sp.ciceri and 7.1 in case of fusarium udum at 30 minutes interval. In case of fusarium oxysporum f.sp.ciceri r2 value is 0.985, 0.984, 0.980 for PG, PMG and Cx. While fusarium udum shows r2 value 0.993 for PG, 0.988 for PMG and 0.966 for Cx. All three enzymes activity increases with increasing time of experiment. Activity of three enzymes are higher in fusarium udum in comparison to fusarium oxysporum f.sp.ciceri. This shows that fusarium udum have higher pathogenic effect.