CALL FOR PAPERS

CERTIFICATE

IMPACT FACTOR 2018

Subject Area

  • Life Sciences / Biology
  • Architecture / Building Management
  • Asian Studies
  • Business & Management
  • Chemistry
  • Computer Science
  • Economics & Finance
  • Engineering / Acoustics
  • Environmental Science
  • Agricultural Sciences
  • Pharmaceutical Sciences
  • General Sciences
  • Materials Science
  • Mathematics
  • Medicine
  • Nanotechnology & Nanoscience
  • Nonlinear Science
  • Chaos & Dynamical Systems
  • Physics
  • Social Sciences & Humanities

Why Us? >>

  • Open Access
  • Peer Reviewed
  • Rapid Publication
  • Life time hosting
  • Free promotion service
  • Free indexing service
  • More citations
  • Search engine friendly

Plagiarism Detection

IJCR is following an instant policy on rejection those received papers with plagiarism rate of more than 20%. So, All of authors and contributors must check their papers before submission to making assurance of following our anti-plagiarism policies.

Pcr amplification by two different primers for early diagnosis of schistosoma mansoni infected snails

Author: 
Pcr amplification by two different primers for early diagnosis of schistosoma mansoni infected snails
Subject Area: 
Life Sciences
Abstract: 

The traditional methods for detection of snail infections cannot differentiate between animals and human Schistosoma and cannot detect early infection so the need for PCR is obligatory. In the present study, two types of primers for conventional PCR were used and compare between them and other traditional methods (shedding, crushing) in attempt to apply this technique in the field for early diagnosis of Schistosoma infection as a trial for better control and prevention of the disease. To fulfil this aim in our study we use two main groups of snails the laboratory groups (L) and the environmental groups (E) collected from water canal in Dakhalia and Sharkiya governorate. All laboratory groups are examined by PCR by using two different primers (121bp, 247bp). All environmental snails are examined for infection by shedding, crushing methods then PCR amplification by two different primers (121bp, 247bp). for detection of infection and the obtained results are compared. DNA was extracted from laboratory infected snails in addition to non infected (as negative control) and subjected to PCR using121 bp primers and 247 bp primers which specific to a partial sequence of S. Mansoni aldolase. The percentage of detection of infection in the laboratory snail were 53,3%, 66,6%, and 73.3%by using of 121bp primer at the 1st, 5rd, and 8thday of infection respectively, however the detection were 86.6%,73.3% and100% by using of 247bp primers. Regarding the environmental snails,on comparing the sensitivity and specificity of crushing method in relation to PCR amplification using 247bp primer in Sharkiya, were found to be 36.3%, 100% respectively while in Dakhalia were found to be 28.5%,100% .the average sensitivity of PCR amplification by 121bp compared to PCR amplification by 247bp was 63.3%. In conclusion, PCR is superior to the conventional methods and can detect positive cases that were negative when examined by shedding or crushing methods. 247bp primer are more specific than 121bp primer for detection of infection in S. Mansoni infected snail and can detect early snail infection and this help control of the disease.

PDF file: 

IJMCE RECOMMENDATION

ONLINE PAYPAL PAYMENT

CURRENT ISSUE

NEWS

CHIEF EDITOR

Rosane Cavalcante Fragoso, Brasil

ASSOCIATE CHIEF EDITOR

   

Jean-Marc SABATIER
Chief Scientific Officer and Head of a Research Group
France

Advantages of IJCR

  • Rapid Publishing
  • Professional publishing practices
  • Indexing in leading database
  • High level of citation
  • High Qualitiy reader base
  • High level author suport

EDITORIAL BOARD

Luai Farhan Zghair
Iraq
Hasan Ali Abed Al-Zu’bi
Jordanian
Fredrick OJIJA
Tanzanian
Firuza M. Tursunkhodjaeva
Uzbekistan
Faraz Ahmed Farooqi
Saudi Arabia
Eric Randy Reyes Politud
Philippines
Elsadig Gasoom FadelAlla Elbashir
Sudan
Eapen, Asha Sarah
United State
Dr.Arun Kumar A
India
Dr. Zafar Iqbal
Pakistan
Dr. SHAHERA S.PATEL
India
Dr. Ruchika Khanna
India
Dr. Recep TAS
Turkey
Dr. Rasha Ali Eldeeb
Egypt
Dr. Pralhad Kanhaiyalal Rahangdale
India
DR. PATRICK D. CERNA
Philippines
Dr. Nicolas Padilla- Raygoza
Mexico
Dr. Mustafa Y. G. Younis
Libiya
Dr. Muhammad shoaib Ahmedani
Saudi Arabia
DR. MUHAMMAD ISMAIL MOHMAND
United State
DR. MAHESH SHIVAJI CHAVAN
India
DR. M. ARUNA
India
Dr. Lim Gee Nee
Malaysia
Dr. Jatinder Pal Singh Chawla
India
DR. IRAM BOKHARI
Pakistan
Dr. FARHAT NAZ RAHMAN
Pakistan
Dr. Devendra kumar Gupta
India
Dr. ASHWANI KUMAR DUBEY
India
Dr. Ali Seidi
Iran
Dr. Achmad Choerudin
Indonesia
Dr Ashok Kumar Verma
India
Thi Mong Diep NGUYEN
France
Dr. Muhammad Akram
Pakistan
Dr. Imran Azad
Oman
Dr. Meenakshi Malik
India
Aseel Hadi Hamzah
Iraq
Anam Bhatti
Malaysia
Md. Amir Hossain
Bangladesh
Ahmet İPEKÇİ
Turkey
Mirzadi Gohari
Iran