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Pcr-based molecular markers for assessment of somaclonal variation and fingerprint varieties in in-vitro cultured prunus spp.

Author: 
Alessia Gargaro, Andrea Frattarelli and Maria Antonietta Palombi
Subject Area: 
Life Sciences
Abstract: 

Tissue culture was used to preserve interesting agronomic traits present in old cultivars of different Prunus spp., maintained in collection at Fruit Trees Research Centre in Rome. Assessment of somaclonal variation and fingerprinting of varieties, using RAPD and SSR analysis, were performed on one-year old plants of Prunus persica (L. Batsch), Prunus armeniaca (L.) and Prunus domestica (L.), in comparison to the mother plant, in vivo maintained. For both of the molecular markers, only reproducible fragments were scored and used to measure genetic similarity by Dice similarity index; similarity estimates were analysed by the UPGMA and the resulting clusters were expressed as dendrograms. The results disclosed genetic variability among the clones analysed from each varieties. Furthermore, while RAPD primers could amplify all DNA samples and can be useful applied to assess genetic variability and fingerprint peach, plum and apricot varieties, SSR primers used, obtained from peach, furnished results which were ambiguous for plum varieties. This last result is also discussed in this report.

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