Pune city is one of the emerging cities of the Modern India. Three major rivers (Pavana, Mula and Mutha) are flowing through this city. All the domestic sewage, industrial effluents and solid waste find its way to these rivers via channels which affect the quality of flowing water and also creating health problems. In the present study an attempt has been made to assess the flowing water pollution in the Pune city. 14 physicochemical parameters were analyzed. Among the two rivers, Mutha was found highly polluted.