The study was carried out at Sam Higginbottom Institute of Agriculture, Technology & Sciences, Allahabad. The aim of the study was to evaluate the physicochemical status of Yamuna water in relation to water pollution in Allahabad. In our study different analytical tests were done such as pH, Temperature, DO, COD, Alkanity, Chlorine, BOD, Total Bacterial count by SPC, TSS and TDS. The results were satisfactory according to the guidelines of Pollution Prevention and Abatement Handbook World Bank Group effectively from July 1998, in which all the standard readings are given.