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Physiological and molecular study on polycystic ovary syndrome in a sample of Iraqi women

Author: 
Rehab Subhi RAMADHAN, Reem Jaafar Ali and Rebah Najah Jabbar AL-gafari
Subject Area: 
Life Sciences
Abstract: 

This study investigates Poly Cystic Ovary Syndrome (PCOS) relation to infertility through a biochemical and molecular at methylene tetrahydrofolate reductase (MTHFR) gene and PCOS gene. Samples from patients suffering PCOS were collected from Kamal al-Samarrai Hospital. Twenty tissue samples were collected from Madinat al-Amamin Al-Kazimin Al-Tibbia Hospital. Fifty blood samples from healthy women served as the control. Average ages of patients and control group were 20-50 years. Subjects were divided in to three age groups (20-30), (31-40) and (41-50), years old. Serum samples for all PCOS patients were measured for fertility hormones levels like Luteinizing Hormone (LH), Follicle Stimulating Hormone FSH and Testosterone hormone. The FSH hormone recorded a significant decreased in age group (41-50) years old, while LH and Testosterone recorded a significant increase in age group (31-40) years old. The study confirmed the incidence of SNPs detected in PCOS gene. Polymerase chain reaction (PCR) was done using a specific primers set to amplify exon (2) of the methylene tetrahydrofolate reductase (MTHFR) gene. Another three primers were designed to amplify the exons (5-10) regions of (PCOS) gene. It was found after PCR product sequencing that the percentage of substitution mutation was 88%, while the deletion mutation percent was 12%.

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