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Phytochemical analysis and pathogenic inhibition activity of pedalium murex (l.) against urinary tract infection bacteria

Author: 
Kaleeswaran Balasubramanian and Ramadevi Shanmugam
Subject Area: 
Life Sciences
Abstract: 

Main objectives of the present work to estimate the phytochemical profile and antibacterial activity of whole plant Pedalium murex (L.) with highly medicinal value against urease producing pathogens. Plants are the effective source of both traditional and recent medicines. Nowadays, medicinal plants were used to treat most unbearable disease among humans such as Urinary tract infections (UTI). The extraction was done by using Traditional Healer Method (THM) and different solvent such as ethanol, methanol, ethyl acetate and petroleum ether by using standard procedures. The antibacterial assay was carried by using agar well method with different Pathogens. The ethyl acetate and petroleum ether extracts of P. murex (L.) (500 µg/ml) showed higher antibacterial activity against gram positive bacteria and gram negative bacteria using micro-dilution method. Minimum Bactericidal Concentration (MBC) and Minimum Inhibitory Concentration (MIC) of ethyl acetate extract showed the value of 625, 62.5, 125, 125 and 15.125 µg/ml against the E. coli, Staphylococcus aureus, Salmonella typhi, Proteus mirablis, Bacillus licheniformis, Bacillus cereus respectively and the petroleum ether extract has 0.39, 12.5, 6.25, 1.56 and 1.56 respectively. MBC value found only in ethyl acetate against S. aureus (>125) and B. cereus (>31.25). From the present work, we conclude that the ethyl acetate and Petroleum ether have potential of antibacterial activity and contain secondary compounds which responsible for biological activities. Due to the presence of phyto-components in the plant extract it may control the bacterial growth either in high concentration/long durations and it may have the ability to control the UTI bacteria.

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