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Phytochemical screening and estimation of total phenols, total flavanoids and evaluation of in vitro anti oxidant and anti inflammatory activities of various extracts of Clitoria ternatea root

Author: 
Neelamma, G., Vanitha, B., Sai Suresh Nehru, Rajesh Kumar and Durai Swamy, B.
Subject Area: 
Health Sciences
Abstract: 

The aim of the present study was to investigate the Phyto -constituents present within the different extracts of Clitoria ternatea root and to estimate total phenols, total flavonoids and anti oxidant, anti-inflammatory activities. Phytochemical studies confirmed the presence of carbohydrates, proteins, alkaloids, glycosides, resins, steroids, saponins, phenols, flavonoids were analyzed using spectro photometric technique, based on Folin - Ciocalteau reagent and aluminium chloride colorimetric assay, respectively. Gallic acid was used as for total phenols and Rutin for total flavonoids. Furthermore, the antioxidant, anti-inflammatory activities were determined by using two in vitro methods namely, 2, 2-phenyl,1-picrylhydazyl (DPPH), Total antioxidant capacity, protein denaturation, HRBC membrane stabilization methods. In these methods, ascorbic acid and Ibuprofen were used as reference compounds. The total phenols and flavonoids were found to be more in methanolic extract. The result clearly indicates that the methanolic extract of Clitoria ternatea Root of the study shows effective antioxidant and anti-inflammatory properties.

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