Preliminary screening and characterization of bifidobacterium from faecal samples

Human faecal samples were used as a source for Bifidobacterium, Gram positive bacteria found in normal microbiota of human beings. There are thirty faecal samples were collected from the new born infants (2- 4 months). Bifidobacterium strains were isolated (MRSc medium), Out of sixty three isolates twenty six showed positivity for bifidus shunt and its species identified based on the biochemical profile. According to the bile and acid tolerance only seven strains were isolated, among this S2 (Bifidobacterium bifidum) showed more resistant capacity against both pH – 2.5 and bile – 0.5% of oxgall (at 4 hrs of treatment).