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A preliminary study of colony forming units of bacteria from the soils of yusmarg forest, kashmir valley India

Author: 
Gowhar Hamid Dar, Azra Kamili N., Ruqeya Nazir, Suhaib Bandh A., and Rouf Ahmad Bhat
Subject Area: 
Physical Sciences and Engineering
Abstract: 

The present study was carried out to get an idea about the bacterial load (density and diversity) and to identify and isolate the bacteria from the soils of Yusmarg forest. A series of dilutions were made from soil samples, from the dilutions, 0.1ml inoculum was poured onto Nutrient agar(NA )and incubated at 28±2oC for 24 hours to study the growth of Bacteria.Comparative analysis of different types of colonies found at the four sites during the study indicates that the bacterial load was dominant in the month of November. The Individual Colony counts of the different types of isolates at the four sites shows that the most dominant isolate type was B8 with a maximum of 73 colonies followed by B25 with 65. The total colony count was maximum at site III (256) followed by site II (198), site I (174) and site IV (144). The total bacterial population was maximum at site III with a cfu/g of 1.8 x 104 and minimum at site I with a cfu/g of 0.7 x 104 in the month of November and in December the maximum bacterial population was found at site 1 with cfu/g of 1.0 x 104 and minimum at site IV with a cfu/g of 0.4 x 104. During the study it was found that isolates B1, B2, B4, B6 and B9 were present only in November while as isolates B15, B21, B25, B27, B28, B29, B30 and B31 were present only in December. However, isolates B7 and B8 were present both in the month of November and December. Among the different isolates obtained from site I B21, B25 and B31 were having maximum cfu/g of 1.5×103 and B4 was having a minimum cfu/g of 0.7×103 and among the different colonies of site II B11 was having maximum cfu/g of 1.6×103 and B26 was having a minimum cfu/g of 0.2×103, similarly among the different colonies of site III B18, B21, B22 and B23 were having a maximum cfu/g of 2×103 and B30 and B33 were having a minimum cfu/g of 0.7×103, while as among the different colonies obtained at site IV B16 and B26 were having a maximum cfu/g of 2×103 and B8, B21 and B31were having a minimum cfu/g of 0.3×103.

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