Study Design and Objective: this was a descriptive cross-sectional study aimed to estimate the prevalence of HIV infection among Sudanese pregnant women at Omdurman Military Hospital. Methods: the Immuno Chromatographic Test (ICT) and the Enzyme-Linked Immunosorbent Assay (ELISA) were used to detect the specific HIV Antigens and antibodies in the serum of 100 asymptomatic pregnant women attended the hospital. Results: only one woman (1%) showed positive result of HIV on both ICT and ELISA. Conclusions: ELISA is more specific than ICT forconfirming the diagnosis of HIV.