Background: Human T-cell Lymphotropic Virus (HTLV) is a retrovirus associated with depression of the immune system. Patients with dual human T-cell lymphotropic virus (HTLV-1) and HIV infections may present with a more serious stage of HIV disease especially at their first medical examination than patients with HIV infection alone, hence the reason for the study. Methodology: The research involved 180 Human Immune Deficiency Virus (HIV) positive individuals. Five (5) mls of blood was collected in EDTA container from each of them and the plasma were tested using ELISA method. Result: The prevalence of HTLV among HIV positive individuals discovered in the study was 0.6 %. The study also revealed that the only positive sample tested was from a 40 year female subject. Conclusion: infection with HTLV poses a great threat to HIV positive individuals whose immunity has already been altered