
Plant Tissue culture has advanced the knowledge of fundamental Biotechnology especially in the field of agriculture, horticulture, plant breeding, forestry, somatic cell hybridization, phytopathology and industrial production of plant metabolites, etc. During the last two decades plant cell, tissue and organ culture have developed rapidly and become a major biotechnological tool in agriculture, horticulture, forestry and industry. That problem which was not feasible through conventional technique, now have been solved via plant tissue culture techniques. In recent years growing of tissue culture banana becoming popular in this area. Banana is an economically important crop, which is extensively cultivated in tropical and subtropical countries. The in-vitro banana plants are superior to the conventional suckers due to their vigorous growth, precocity and higher yields. An attempt was made to standardize the production of tissue cultured banana (Robusta sp) compare to conventional sucker propagation method.