Nitrogen has been the most essential element that directly affects the growth of plants. It is present in the soil in insoluble form which cannot be used by the plants. The plants require soluble form of nitrogen in soil to grow and sustainability. One such important compound that provides the plant with required nitrogen is Amino Acid (protein). It can be extracted from different plant or animal matter. Soya bean is one such source of protein and due to its high protein yields, it can be used to extract protein by three different methods such as acid hydrolysis, alkali hydrolysis and with the help of enzymes. Protein can be used to regulate the Carbon-Nitrogen ratio of the soil. This C-N ratio if present in 24:1, is optimal for the soil microbes to convert nutrients like Nitrogen, Potassium and Zinc in usable form for the crops.