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quantification of sulbactum sodium and ceftriaxone sodium in parenteral dosage form by novel lc method

Author: 
Srikanth, I., Neeraja Kamakshi, U., Niveditha, R. and Ratna Kumari, P.
Subject Area: 
Health Sciences
Abstract: 

A simple, sensitive and precise reverse phase high performance liquid chromatographic method has been developed for the estimation of Sulbactum sodium (SBS) and Ceftriaxone sodium (CTS) in pharmaceutical dosage forms. The RP-HPLC, separation was carried out by using Agilent XDB, C18 (150 x 4.6 mm, 5m) analytical column and detection was carried out at 218nm by using variable wavelength detector. The mobile phase consists of buffer (0.02 M Sodium Dihydrogen phosphate, pH-3 was adjusted with ortho phosphoric acid): Acetonitrile in the ratio of 82:18 % v/v delivered at a flow rate of 1.0 ml / min and wavelength of detection at 218nm. The retention times of SBS and CTS were found to be 2.375 and 2.989 min respectively. The developed method was validated according to ICH guidelines. The results indicates that the method was found to be simple, rapid, precise and accurate and can be adopted to routine methods of analysis of SBS and CTS in Pharmaceutical dosage forms.

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