Rapid and efficient plant regeneration from shoot tip explants of orthosiphon spiralis murr- An important medicinal plant

An efficient plant regeneration protocol was developed for Orthosiphon spiralis - an important medicinal plant. Shoot tip explants inoculated on Murashige and Skoog (MS) medium supplemented with 1.0 mg l-1 6- Benzyl adenine (BAP) and 0.25 mg l-1 NAA showed better growth response and produced 52.3 ± 2.1 shoots per explant with an average length of 6.43 ± 0.06 cm after 30 days. Rooting of shoots was achieved on growth regulator free half strength MS medium produced 7.3 ± 0.25 cm roots with an average height of 5.4 ± 0.2 cm after 28days. The rooted plantlets were transferred for hardening, 80 per cent of plantlets survived and resumed growth in the mixture of soil, vermiculite and farmyard manure (1:1:1).