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Relative expression of slow growth suppressor gene(SGS1) under the treatment of herbal formulation on yeast model

Author: 
Sudhakar Malla, Selvam Arjunan, Sanjana, W.K., Anu John, B., Gunna Vidyanandh and Shriramwar Yogesh Gajanan
Subject Area: 
Life Sciences
Abstract: 

Hair loss is one the dermatological disorder, estimated to effect nearly 2% of the worlds population. The problem of hair loss, greying of hair in young age is normally caused due to exposure to pollution, pesticides, imbalanced nutrition, altered gene expression and also harmful chemicals that are present in dermatological products. The present study was aimed to evaluate the expression of SGS1 gene which is homologue to human WRN and BLM gene that plays an important role in hair follicle development. The hair growth activity was studied by treating the yeast cells with herbal extracts (Eclipta alba and Ficus Bengalensis prop roots) and simultaneously inducing the cells to stress by organopesticides to investigate the inhibitory action of the herbal extracts on yeast cells. The cell viability and antioxidant assays were performed for the herbal extracts where significant results were observed in ethanol and methanol fractions. The expression of SGS1 gene was studied using RT-PCR, where the expression of the gene was found to be lowered in case of yeast cells treated with pesticides whereas the gene expression was found to be more in yeast cells that were treated with pesticides and herbal extracts. However further study at protein level is needed to conform the activity with the pure extract.

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